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#1 Posted : 25 September 2008 16:35:00(UTC)
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Posted By Crim
I read in a newsletter today that a man opening a bag of compost at home in his garden inhaled bio spores contained within the bag and it led to his death. The article was directed at waste recycling companies that deal with garden waste for composting.

The article went on to say that we all breathe in bio spores on a daily basis in gardens, parks etc. but usually on a small dose level.

Not knowing anything about Bio spores can someone please throw some light on the subject?

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#2 Posted : 25 September 2008 16:57:00(UTC)
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Posted By Colin Reeves
Check out the following - seems to have a grain of truth in the story.

http://www.dailymail.co....ease-garden-compost.html

http://www.rsc.org/Membe...onellaConference2006.asp

Colin
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#3 Posted : 25 September 2008 17:56:00(UTC)
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Posted By Chris Packham
With many bacteria it isn't the bacteria themselves that are the real hazard but either spores or what are termed endotoxins.

For example, botulitis is a very nasty form of food poisoning. It is caused not by the botulinum bacteria directly but by the endotoxins that they release when they die. Endotoxins are often the real problem with water-mixed metalworking fluids, not the actual bacteria in the fluid. These may break the fluid down or cause that nasty smell as they die, but it is what they leave behind that, if it can get into the body (inhalation, cuts, etc.), causes the health problem.

With C.difficile it is the spores that are the problem. Whilst outside the body the bacteria will die relatively quickly, spores that they generate can remain viable for years on surfaces such as in a toilet, in a hospital ward, etc. When conditions are right, e.g. from ingestion, they become active and cause the infection. Incidentally, spores are not generally killed by antiseptics.

Bacteria in compost can generate spores. Normally these will not be in sufficient concentration to represent a hazard, but if the compost were to be exceptionally dusty and a significant volume of dust inhaled, then there could be a risk of damage to health. Keep in mind also that some people will be more susceptible than others, so one may not suffer any ill effect whilst another could become quite ill.

Chris

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#4 Posted : 25 September 2008 19:25:00(UTC)
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Posted By Ian Blenkharn
As it says in the heading of these forum pages 'Postings made by forum users should not be relied upon in making or refraining from making any decision.'

That is very good advice. What I read above is hopelessly incorrect.

Ian Blenkharn
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#5 Posted : 25 September 2008 19:33:00(UTC)
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Posted By Crim
Colin, Chris, thanks, try this link as I think it's the one I heard about?

http://www.mirror.co.uk/...-compost-89520-20605487/

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#6 Posted : 25 September 2008 20:41:00(UTC)
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Posted By Chris Packham
Ian

You state that what is written is incorrect. Would you care to justify that statement? For the record I have been working recently very closely with microbiologists on this topic as part of an involvement with cross-infection in hospitals, so your views would be of interest.

Chris
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#7 Posted : 26 September 2008 15:04:00(UTC)
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Posted By Ian Blenkharn
Infections and other adverse health effects – mainly allergic – that are associated with composting are generally linked to a high fungal biomass. Indeed, aspergillosis was identified in the report mentioned by the original correspondent making your discourse on matters bacteriological somewhat redundant.

Legionella have been reported in this situation, albeit rarely. So rare in fact that it leads you far off target. Your focus on bacteria rather than fungi brings mention of both spores and endotoxin, though you should know that the two are mutually exclusive. Sporulating Gram positive species have a fundamentally different cell wall structure and composition to the endotoxin-rich cell walls of Gram negative species. And take care not to confuse bacterial and fungal spores.

Next you turn to “botulitis”. You might know the name of the organism to which you refer, and of the disease process, botulism, that it can cause. But it is Clostridium botulinum that causes botulism. That inaccuracy alone gives me cause for concern. Don’t blame the spell checker.

And just what causes botulism? With few exceptions, ingestion of pre-formed botulinum toxin. That toxin is an exotoxin, not an endotoxin. It is nothing like an endotoxin, in origin, in structure, in composition, or in biological activity. The distinction is not just a couple of letters that slip though a spell checker, but is far more profound. Think chalk and cheese.

From here you jump to metalworking fluids, though I’m not clear how you perceive the link from composing to legionnaires disease, to botulism, to MWF. With MWF, attribute the cause of a diverse range of symptoms from simple dermatitis to asthma to severe and potentially debilitating extrinsic allergic alveolitis, to the presence in MWF of endotoxins. There is limited evidence for this, though that evidence is not strong. Indeed, it may be flawed, at least in part, as many affected individuals will show an immunological response to mycobacterial and other Gram positive cell wall fractions. Endotoxins may be significant in some cases, but such sweeping generalisations ignore, or reveal a lack of understanding of, a large body of highly complex information. The published data, the facts, go back as far as the early 1950s. It makes interesting reading.
As an aside, you tell us that endotoxins are liberated as bacteria die. That is true, but of course relates only to Gram negative species. Your additional comment that inhalation of endotoxins can be associated with respiratory systems is equally true, but the comment about endotoxins in cuts is risible.

And now to the spore-forming bacterium Clostridium difficile. Here you make a couple of points with which I would not disagree, though in what way you believe that information might have anything to do with the issue at hand simply defies logic.

Then straight back to compost, where you focus again on the possibility that within it bacteria can generate spores. That is indeed true but you link that, quantitatively, with a possible hazard. Where is the evidence? It does not exist. Not legionnaires (that’s a Gram negative non-spore forming species), not aspergillosis (that’s a fungus, one of several that are now recognised as potentially harmful in this situation). Not antibiotic-associated diarrhoea or pseudomembranous colitis caused by C difficile. And nothing to do with bacterial endotoxins, or for that matter with bacterial exotoxins either.

It really is important to stick to what you know. It is equally important for those who seek information in this place to satisfy themselves that what they read has some basis in fact, and is neither misleading nor simply wrong.

Being positive, this is all about knowledge, the depth and breadth of that knowledge, how that knowledge is presented, and an understanding of its limitations. Helping others with fact is a laudable intention, and expressions of opinion make for lively discussion and a stimulating exchange of views. There must however be some checks and controls. The moderators, in their rather thankless task, deal admirably with many issues but cannot be expected to judge on the quality of badly muddled and in part hopelessly incorrect information in specialist areas. As an experienced microbiologist with a substantial research-led background in almost every one of the areas through which you chose to wander, I thought it important to point out to others that they should take caution. That view has not changed.

Saying that you have recently worked closely with microbiologists tells me enough. It tells me that you are not a microbiologist, and that you should not consider working alongside one as sufficient to impart any experience or understanding of the subject(s). I work alongside many experienced professionals but would not believe for one moment that that gives me a proper knowledge of their respective specialist subjects.

You asked for an explanation. I hope that you can accept that explanation, presented in a way that provides to others an awareness of the issues involved and the need to ensure that what they read is actually worth reading.
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