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ttxela  
#1 Posted : 25 August 2016 14:42:35(UTC)
Rank: Forum user
ttxela

We're looking at the possibility of using proprietary UV sterilisation lamps for certain lab areas. These units have a delay timer so you can set them then leave the room whilst it works for a set period. We can use signage and perhaps lock the door to prevent inadvertent exposure when the lamp is lit however I'm concerned about the vision panels into the room and access by cleaners/security out of hours. Does anyone else use these units?
Jane Blunt  
#2 Posted : 25 August 2016 14:52:15(UTC)
Rank: Super forum user
Jane Blunt

I suggest locking the door to prevent access by anyone during the sterilisation cycle. The vision panels would then be redundant and you could cover them. Depending on the wavelength of the UV, they may not transmit much of it. Bear in mind that UV light can damage some materials.
peterL  
#3 Posted : 25 August 2016 15:53:09(UTC)
Rank: Forum user
peterL

Hi, Why not consider an ozone generator, as an alternative. Pete,
Jane Blunt  
#4 Posted : 25 August 2016 18:03:06(UTC)
Rank: Super forum user
Jane Blunt

There are pros and cons to both. Ozone also damages some materials and it is a gas so could potentially affect the whole room. If needed, UV could be directed at quite a small area of the room and the potential damage would be limited to that area. UV can be switched off and is instantly rendered safe. Ozone will persist until it has all degraded into oxygen. I believe that can take quite a long time in clean air. (It only takes a few minutes in heavily polluted air). Ozone is toxic. http://www.hse.gov.uk/pubns/eh38.pdf
toe  
#5 Posted : 25 August 2016 23:03:59(UTC)
Rank: Super forum user
toe

What about a door switch that turns the lamps off when opened.
ttxela  
#6 Posted : 26 August 2016 09:28:57(UTC)
Rank: Forum user
ttxela

Well, the intention is to destroy DNA which I don't think ozone would do, so it has to be UV I think. At the moment a small UV cabinet is used but there is a desire to do the whole room. We'll probably end up relying on securing the room and covering windows however I was wondering about some sort of PIR that would switch the lamp off if someone forgot to lock the door etc.
ttxela  
#7 Posted : 26 August 2016 09:37:07(UTC)
Rank: Forum user
ttxela

This is the sort of thing we are considering; http://www.uvc-lightprod...-mobile-room-steriliser/ I was hoping someone else used something similar. Perhaps I'm a little over cautious having had issues with UV before.
Jane Blunt  
#8 Posted : 26 August 2016 10:59:52(UTC)
Rank: Super forum user
Jane Blunt

In your quest to sterilise the whole room I think you need to explore the effectiveness of this device. Does it sterilise surfaces that are not in a line of sight of the lamp? I suspect it might not. Light of long wavelengths (infrared) is reflected by matt walls, but I am not sure about short wavelengths. I would have thought that ozone would destroy DNA as it is a very powerful oxidising agent. It is worth investigating if you want to sterilise the whole room.
A Kurdziel  
#9 Posted : 26 August 2016 12:23:15(UTC)
Rank: Super forum user
A Kurdziel

I have used this in a past life but you need to be careful, definitely lock the door when the UV is on and get an interlock to turn off the UV when the door is opened. UV is invisible and it is not always obvious when the lights are on, which is when you get burned (I know from personal experience). Overall I think it is safer than ozone and cheaper but not forget that the UV tubes degrade over time and although they seem to be working the amount of UV light they emit falls off and they become ineffective. Do not assume that they sterilise the lab: it’s still a lab (at CL2 I would assume) and this is no substitution for good bench top hygiene.
johnmurray  
#10 Posted : 26 August 2016 18:29:34(UTC)
Rank: Super forum user
johnmurray

Jane Blunt wrote:
In your quest to sterilise the whole room I think you need to explore the effectiveness of this device. Does it sterilise surfaces that are not in a line of sight of the lamp? I suspect it might not. Light of long wavelengths (infrared) is reflected by matt walls, but I am not sure about short wavelengths. I would have thought that ozone would destroy DNA as it is a very powerful oxidising agent. It is worth investigating if you want to sterilise the whole room.
I think you'll find IR is absorbed by Matt surfaces and re-emitted. UV is not reflected by Matt surfaces to any great extent, unless you use white! The sterilising effect of UV is also affected by distance from the area to be treated.
Jane Blunt  
#11 Posted : 26 August 2016 19:16:36(UTC)
Rank: Super forum user
Jane Blunt

johnmurray wrote:
Jane Blunt wrote:
In your quest to sterilise the whole room I think you need to explore the effectiveness of this device. Does it sterilise surfaces that are not in a line of sight of the lamp? I suspect it might not. Light of long wavelengths (infrared) is reflected by matt walls, but I am not sure about short wavelengths.
I think you'll find IR is absorbed by Matt surfaces and re-emitted. UV is not reflected by Matt surfaces to any great extent, unless you use white! The sterilising effect of UV is also affected by distance from the area to be treated.
What I had in mind was that you get specular reflection from surfaces where the imperfections are smaller than the wavelength of the light. Since IR has longer wavelengths, surfaces that are matt in the visible range might become mirrors in the IR. Anyway, I doubt if the UV reflects well from most surfaces, so I would expect it to only sterilise effectively in the line of sight of the lamp.
johnmurray  
#12 Posted : 30 August 2016 09:58:30(UTC)
Rank: Super forum user
johnmurray

Jane Blunt wrote:
johnmurray wrote:
Jane Blunt wrote:
In your quest to sterilise the whole room I think you need to explore the effectiveness of this device. Does it sterilise surfaces that are not in a line of sight of the lamp? I suspect it might not. Light of long wavelengths (infrared) is reflected by matt walls, but I am not sure about short wavelengths.
I think you'll find IR is absorbed by Matt surfaces and re-emitted. UV is not reflected by Matt surfaces to any great extent, unless you use white! The sterilising effect of UV is also affected by distance from the area to be treated.
What I had in mind was that you get specular reflection from surfaces where the imperfections are smaller than the wavelength of the light. Since IR has longer wavelengths, surfaces that are matt in the visible range might become mirrors in the IR. Anyway, I doubt if the UV reflects well from most surfaces, so I would expect it to only sterilise effectively in the line of sight of the lamp.
I think you'll find that most "reflection" at IR by surfaces is re-emission. UV sterilisation is UV at 100-300 nm (ish) and that range is also strongly absorbed by atmosphere, so to sterilise air effectively some sort of circulation may need to be arranged. It may be better to arrange a low humidity as well? Ok on the line of sight.... What the OP needs is a strongly emitting gamma source!! Or: Nuke it from orbit. It's the only way to be sure!
A Kurdziel  
#13 Posted : 30 August 2016 12:14:01(UTC)
Rank: Super forum user
A Kurdziel

UV is used principally as a means to disable DNA rather than to actually sterilise the lab. It has some effect of virus but less than a good disinfectant. As Jane Blunt said the UV will only work in line of sight of the UV lamps but hopefully who ever worked in the lab wasn’t splashing the nucleic acids about. The main reason for controlling the nucleic acids is a matter of quality control. A single stray DNA molecule can really screw up your experiments, so it is worth doing this alongside more traditional disinfection/cleaning methods. The controls which I mentioned-signage, interlocking switches and doors etc are fairly well established procedures in this area.
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